業績

 

英文原著

2019年

Morii I, Iwabuchi Y, Mori S, Suekuni M, Natsume T, Yoshida KSugimoto N, Kanemaki MT, Fujita M. Inhibiting the MCM8-9 complex selectively sensitizes cancer cells to cisplatin and olaparib. Cancer Sci., 110: 1044-1053, 2019. (Selected for the Issue Highlights)


2018年

Kondo, Y., Higa, S., Iwasaki, T., Matsumoto, T., Maehara, K., Harada, A., Baba, Y., Fujita, M., Sato, Y., Ohkawa, Y. Sensitive detection of fluorescence in western blotting by merging images. PLoS One, DOI:10.1371/journal.pone.0191532, 2018.


Watanabe S, Fujiyama H, Takafuji T, Kayama K, Matsumoto M, Nakayama KI, Yoshida KSugimoto N, Fujita M. GRWD1 regulates ribosomal protein L23 levels via the ubiquitin-proteasome system. J. Cell Sci., DOI: 10.1242/jcs. 213009., 2018.


Sugimoto N, Maehara K, Yoshida K, Ohkawa Y, Fujita M. Genome-wide analysis of the spatiotemporal regulation of firing and dormant replication origins in human cells. Nucleic Acids Res., 46: 6683-6696, 2018.


2017年

Yoshimatsu, Y., Nakahara, T., Tanaka, K., Inagawa, Y., Narisawa-Saito, M., Yugawa, T., Ohno, Sl., Fujita, M., Nakagama, H., Kiyono, T. Roles of the PDZ-binding motif of HPV 16 E6 protein in oncogenic transformation of human cervical keratinocytes. Cancer Sci.,108(7):1303-1309, 2017


Higa, M., Kushiyama, T., Kurashige, S., Kohmon, D., Enokitani, K., Iwahori, S., Sugimoto, N., Yoshida, K., Fujita, M. TRF2 recruits ORC through TRFH domain dimerization. Biochimica et Biophysica Acta, 1864: 191-201, 2017.


Kayama,K., Watanabe,S., Takafuji,T., Tsuji,T., Hironaka,K., Matsumoto,M., Nakayama,K., Enari,M., Kohno, T., Shiraishi,K., Kiyono,T., Yoshida,K., Sugimoto,N., Fujita,M. GRWD1 negatively regulates p53 via the RPL11–MDM2 pathway and promotes tumorigenesis. EMBO Rep., 18: 123-137, 2017.


2016年

Kuniyoshi, Y.,Maehara, K., Iwasaki, T.,  Hayashi, M.,  Semba, Y., Fujita, M., Sato, Y.,  Kimura, H.,  Harada, A.,  Ohkawa, Y. Identification of Immunoglobulin Gene Sequences from a Small Read Number of mRNA-Seq Using Hybridomas. PLoS One, DOI:10.1371/journal.pone.0165473, 2016.


Aizawa, M., Sugimoto, N., Watanabe, S., Yoshida, K. and Fujita, M.  Nucleosome assembly and disassembly activity of GRWD1, a novel Cdt1-binding protein that promotes pre-replication complex formation. Biochimica et Biophysica Acta, 1863: 2739-2748, 2016.


2015年

Sugimoto, N., Maehara, K., Yoshida, K., Yasukoushi, S., Osano, S., Watanabe, S., Aizawa, M., Yugawa, T., Kiyono, T., Kurumizaka, H., Ohkawa, Y. and Fujita, M.: Cdt1-binding protein GRWD1 is a novel histone-binding protein that facilitates MCM loading through its influence on chromatin architecture.  Nucleic Acids Res., 43: 5898-5911, 2015.


Ohira, M., Iwasaki, Y., Tanaka, C., Kuroki, M., Mastsuo, N., Kitamura, T., Yukuhiro, M., Morimoto, H., Pang, N., Liu, B., Kiyono, T., Amemiya, M., Tanaka, K., Yoshida, K., Sugimoto, N., Ohshima, T. and Fujita, M.: A novel anti-microtuble agent with carbazole and benzohydrazide structures suppresses tumor cell growth in vivo.  Biochimica et Biophysica Acta, 1850: 1676-1684, 2015.


2014年

Yoshida, K., Bacal, J., Desmarais, D., Padioleau, I., Tsaponia, O., Chabes, A., Pantesco, V., Dubois, E., Parrinello, H., Skrzypczak, M., Ginalski, K., Lengronne, A. and Pasero, P.: The Histone Deacethlases Sir2 and Rpd3 Act on Ribosomal DNA to Control the Replication Program in Budding Yeast.  Mol. Cell, 54: 691-697, 2014.


Iwahori, S., Kohmon, D., Kobayashi, J., Tani, Y., Yugawa, T., Komatsu, K., Kiyono, T., Sugimoto, N., and Fujita, M.: ATM regulates Cdt1 stability during the unperturbed S phase to prevent re-replication.  Cell Cycle,13,471-481,2014. (Selected for “News&Views”)


2013年

Yugawa, T., Nishino, K., Ohno, S., Nakahara, T., Fujita, M., Goshima, N., Umezawa, A., and Kiyono, T.: Non-canonical Notch signaling limits self-renewal of human epithelial and iPS cells through ROCK activation.   Mol. Cell. Biol. 33: 4434-4447, 2013.


2012年

Egawa, N., Nakahara, T., Ohno, S., Narisawa-Saito, M., Yugawa, T., Fujita, M., Yamato, K., Natori, Y. and Kiyono, T.: The E1 protein of human papillomavirus type 16 is dispensable for maintenance replication of the viral genome.  J. Virol. 86: 3276-3281, 2012.


Nishimoto, N., Watanabe, M., Watanabe, S., Sugimoto, N., Yugawa, T., Ikura, T., Koiwai, O., Kiyono,T. and Fujita, M.: Heterocomplex formation by Arp4 and β-actin involved in integrity of the Brg1 chromatin remodeling complex.  J. Cell Sci.,125:3870-82,2012.(Selected for “In This Issue” and “Cover Page”)


Narisawa-Saito, M., Inagawa, Y., Yoshimatsu, Y., Haga, K., Tanaka, K., Egawa, N., Ohno, S., Ichikawa, H., Yugawa, T., Fujita,  M. and Kiyono, T.: A critical role of MYC for transformation of human cells by HPV16 E6E7 and oncogenic HRAS.  Carcinogenesis, 33:910-7,2012.


2011年

Sugimoto N, Yugawa T, Iizuka M, Kiyono T, Fujita M. Chromatin remodeler sucrose nonfermenting 2 homolog (SNF2H) is recruited onto DNA replication origins through interaction with Cdc10 protein-dependent transcript 1 (Cdt1) and promotes pre-replication complex formation. J. Biol. Chem., 286: 39200-39210, 2011.


Zushi Y, Narisawa-Saito M, Noguchi K, Yuki Y, Yugawa T, Egawa N, Fujita M, Urabe M, Kiyono T.  An in vitro multistep carcinogenesis model for both HPV-positive and -negative human oral squamous cell carcinomas. Am. J. Cancer Res., 1: 869-881, 2011.


2010年

Yoshida K, Sugimoto N, Iwahori S, Yugawa T, Narisawa-Saito M, Kiyono T, Fujita M.  CDC6 interaction with ATR regulates activation of a replication checkpoint in higher eukaryotic cells. J. Cell Sci., 123: 225-235, 2010.


Yugawa T, Narisawa-Saito M, Yoshimatsu Y, Haga K, Ohno S, Egawa N, Fujita M, Kiyono T. DeltaNp63alpha repression of the Notch1 gene supports the proliferative capacity of normal human keratinocytes and cervical cancer cells. Cancer Res., 70: 4032-4044, 2010.


2009年

Sugimoto N, Yoshida K, Tatsumi Y, Yugawa T, Narisawa-Saito M, Waga S., Kiyono T, Fujita M.  Redundant and differential regulation of multiple licensing factors ensures prevention of rereplication in normal human cells.  J. Cell Sci., 122: 1184-1191, 2009.


Iizuka M, Takahashi Y, Mizzen CA, Cook RG, Fujita, M, Allis CD, Frierson Jr HF, Fukusato T, Smith MM.  Histone acetyltransferase Hbo1: catalytic activity, cellular abundance, and links to primary cancers. Gene, 436: 108-114, 2009.


Sasaki, R., Narisawa-Saito, M., Yugawa, T., Fujita M., Tashiro, H., Katabuchi, H. and Kiyono, T.: Oncogenic transformation of human ovarian surface epithelial cells with defined cellular oncogenes.  Carcinogenesis 30: 423-431, 2009.


2008年

Tatsumi Y, Ezura K, Yoshida K, Yugawa T, Narisawa-Saito M, Kiyono T, Ohta S, Obuse C, Fujita M.  Involvement of human ORC and TRF2 in pre-replication complex assembly at telomeres.  Genes Cells, 13: 1045-1059, 2008.


Sugimoto N, Kitabayashi I, Osano S, Tatsumi Y, Yugawa T, Narisawa-Saito M, Matsukage A, Kiyono T, Fujita M. Identification of novel human Cdt1-binding proteins by a proteomics approach: Proteolytic regulation by APC/CCdh1.  Mol. Biol. Cell, 19: 1007-1021, 2008.


Mizushina Y, Takeuchi T, Hada T, Maeda N, Sugawara F, Yoshida H, Fujita M.  The inhibitory action of SQDG (sulfoquinovosyl diacylglycerol) from spinach on Cdt1-geminin interaction.  Biochimie, 90: 947-956, 2008.


Narisawa-Saito M, Yoshimatsu Y, Ohno S, Yugawa T, Egawa N, Fujita M, Hirohashi S, Kiyono, T.  An in vitro multistep carcinogenesis model for human cervical cancer.  Cancer Res., 68: 5699-5705, 2008.


Mizushina Y, Takeuchi T, Takagusagi Y, Yonezwa Y, Mizuno T, Yanagi K, Imamoto N, Sugawara F, Sakaguchi K, Yoshida H, Fujita, M.  Coenzyme Q10 as a potent compound that inhibits Cdt1-geminin interaction.  Biochim. Biophy. Acta., 1780: 203-213, 2008.


2007年

Yugawa T, Handa K, Narisawa-Saito M, Ohno S, Fujita M, Kiyono T. Regulation of Notch1 gene expression by p53 in epithelial cells.  Mol. Cell. Biol., 27: 3732-3742, 2007.


Narisawa-Saito M, Handa K, Yugawa T, Ohno S, Fujita M, Kiyono T.  HPV16 E6-mediated stabilization of ErbB2 in neoplastic transformation of human cervical keratinocytes.  Oncogene, 26: 2988-2996, 2007.


Handa K, Yugawa T, Narisawa-Saito M, Fujita M, Kiyono T. E6AP-dependent degradation of DLG4/PSD95 by high-risk human papillomavirus type 18 E6 protein. J. Virol., 81: 1379-1389, 2007.


2006年

Tatsumi Y, Sugimoto N, Yugawa T, Narisawa-Saito M, Kiyono T, Fujita M. Deregulation of Cdt1 induces chromosomal damage without rereplication and leads to chromosomal instability. J. Cell Sci., 119: 3128-3140, 2006.


Nishitani H, Sugimoto N, Roukos V, Nakanishi Y, Saijo M, Obuse C, Tsurimoto T, Nakayama K-I, Nakayama K, Fujita M, Lygerous Z, Nishimoto T. Two E3 ubiquitin ligases, SCF-Skp2 and DDB1-Cul4, target human Cdt1 for proteolysis.  EMBO J., 25: 1126-1136, 2006.


2005年

Shirata N, Kudoh A, Daikoku T, Tatsumi Y, Fujita M, Kiyono T, Sugaya Y, Isomura H, Ishizaki K, Tsurumi T. Activation of ATM DNA damage checkpoint signal transduction elicited by Herpes simplex virus infection.  J. Biol. Chem., 280: 30336-30341, 2005.


Daikoku T, Kudoh A, Fujita M, Sugaya Y, Isomura H, Shirata N, Tsurumi T. Architecture of replication compartments formed during Epstein-Barr Virus lytic replication. J. Virol., 79: 3409-3418, 2005.


Kudoh A, Fujita M, Zhan L, Shirata N, Daikoku T, Sugaya Y, Isomura H, Nishiyama Y, Tsurumi T. Epstein-Barr virus lytic replication elicits ATM checkpoint signal transduction while providing an S-phase-like cellular environment.  J. Biol. Chem., 280: 8156-8163, 2005.


2004年

Sugimoto N, Tatsumi Y, Tsurumi T, Matsukage A, Kiyono T, Nishitani H, Fujita M.  Cdt1 phosphorylation by cyclin A-dependent kinases negatively regulates its function without affecting geminin binding.  J. Biol. Chem., 279: 19691-19697, 2004.


Daikoku T, Kudoh A, Fujita M, Sugaya Y, Isomura H, Tsurumi T. In vivo dynamics of EBNA 1-Ori P interaction during latent and lytic replication of Epstein-Barr virus.  J. Biol. Chem., 279: 54817-54825, 2004.


Kudoh A, Daikoku T, Sugaya Y, Isomura H, Fujita M, Kiyono T, Nishiyama Y, Tsurumi T. Inhibition of S-phase cyclin-dependent kinase activity blocks expression of Epstein-Barr virus immediate-early and early genes, preventing viral lytic replication.  J. Virol., 78: 104-115, 2004.


2003年

Fujita M, Ichinose S, Kiyono T, Tsurumi T, Omori A. Establishment of latrunculin A-resistance in HeLa cells by expression of R183A D184A mutant α-actin. Oncogene, 22: 627-631, 2003.


Ohta S, Tatsumi Y, Fujita M, Tsurimoto T, Obuse, C. The ORC1 cycle in human cells: II. Dynamic change of human ORC complex during the cell cycle.  J. Biol. Chem., 278: 41535-41540, 2003.


Kudoh A, Fujita M, Kiyono T, Kuzushima K, Sugaya Y, Izuta S, Nishiyama Y, Tsurumi T. Reactivation of lytic replication from Epstein-Barr virus latently infected B-cells occurs with high S-phase CDK activity while inhibiting cellular DNA replication.  J. Virol., 77: 851-861, 2003.


2002年

Fujita M, Ishimi Y, Nakamura H, Kiyono T, Tsurumi T. Nuclear organization of DNA replication initiation proteins in mammalian cells. J. Biol. Chem., 277: 10354-10361, 2002.


2000年以前

Yokoyama N, Hirata M, Ohtsuka K, Fujii K, Fujita M, Kuzushima K, Kiyono T, Tsurumi T. Co-expression of human chaperone Hsp70 and Hsdj or Hsp40 co-factor increases solubility of overexpressed target proteins. Biochim. Biophys. Acta., 1493: 119-124, 2000.


Fujii K, Yokoyama N, Kiyono T, Kuzushima K, Homma M, Nishiyama Y, Fujita M, Tsurumi T. The Epstein-Barr virus pol catalytic subunit physically interacts with the BBLF4/BSLF1/BBLF2/3 complex. J. Virol., 74: 2550-2557, 2000.


Arata Y, Fujita M, Ohtani K, Kijima S, Kato J. Cdk2-dependent and -independent pathways in E2F-mediated S phase induction.  J. Biol. Chem., 275: 6337-6345, 2000.


Fujita M, Yamada C, Goto H, Yokoyama N, Kuzushima K, Inagaki M, Tsurumi T. Cell cycle regulation of human CDC6 protein: intracellular localization, interaction with the human MCM complex and CDC2 kinase-mediated hyperphosphorylation.  J. Biol. Chem., 274: 25927-25932, 1999.


Goto H, Tomono Y, Ajiro K, Kosako H, Fujita M, Sakurai M, Okigaki T, Takahashi T, Inagaki M. Identification of a novel phosphorylation site on histone H3 coupled with mitotic chromosome condensation.  J. Biol. Chem., 274: 25543-25549, 1999.


Kuzushima K, Hoshino Y, Fujii K, Yokoyama N, Fujita M, Kiyono T, Kimura H, Morishima T, Morishima Y, Tsurumi T. Rapid determination of Epstein-Barr virus-specific CD8+ T cell frequencies by flow cytometry. Blood, 94: 3094-3100, 1999.


Kuzushima K, Nakamura S, Nakamura T, Yamamura Y, Yokayama N, Fujita M, Kiyono T, Tsurumi T. Increased frequency of antigen-specific CD8+ cytotoxic T lymphocytes infiltrating an EBV-associated gastric carcinoma. J. Clin. Invest., 104: 163-171, 1999.


Kosako H, Goto H, Yanagida M, Matsuzawa K, Fujita M, Tomono Y, Okigaki T, Odai H, Kaibuchi K, Inagaki M. Specific accumulation of Rho-associated kinase at the cleavage furrow during cytokinesis: cleavage furrow-specific phosphorylation of intermediate filaments. Oncogene, 18: 2783-2788, 1999.


Fujita M, Yamada C, Tsurumi T, Hanaoka F, Matsuzawa K, Inagaki M. Cell cycle- and chromatin binding state-dependent phosphorylation of human MCM heterohexameric complexes: a role for cdc2 kinase.  J. Biol. Chem., 273: 17095-17101, 1998.


Fujita M, Kiyono T, Hayashi Y, Ishibashi M. In vivo interaction of human MCM heterohexameric complexes with chromatin: possible involvement of ATP. J. Biol. Chem., 272: 10928-10935, 1997.


Kiyono T, Hiraiwa A, Fujita M, Hayashi Y, Akiyama T, Ishibashi M. Binding of high risk HPV E6 oncoproteins to the human homologue of the Drosophila discs large tumor suppressor protein.  Proc. Natl. Acad. Sci. USA, 94: 11612-11616, 1997.


Hiraiwa A, Fujita M, Nagasaka T, Adachi A, Ohashi M, Ishibashi M. Immunolocalization of hCDC47 protein in normal and neoplastic human tissues and its relation to growth. Int. J. Cancer, 74: 180-184, 1997.


Fujita M, Kiyono T, Hayashi Y, Ishibashi M. hCDC47, a human member of the MCM family: dissociation of the nucleus-bound form during S phase.  J. Biol. Chem., 271: 4349-4354, 1996.


Fujita M, Kiyono T, Hayashi Y, Ishibashi M. Inhibition of S phase entry of human fibroblasts by an antisense oligomer against hCDC47.  Biochem. Biophys. Res. Commun., 219: 604-607, 1996.


Murata K, Fujita, M, Honda T, Yamada Y, Tomonaga M, Shiku H. Rat primary T cells expressing human T-lymphotropic virus type I tax gene transduced by a retroviral vector: in vitro and in vivo characterization.  Int. J. Cancer, 68: 102-108, 1996.


Fujita M, Shiku H. Differences in sensitivity to induction of apoptosis among rat fibroblast cells transformed by HTLV-I tax gene or cellular nuclear oncogenes.  Oncogene, 11: 15-20, 1995.


Ido A, Nakata K, Kato Y, Nakao K, Murata K, Fujita M, Ishii N, Tamaoki T, Shiku H, Nagataki S. Gene therapy for hepatoma cells using a retrovirus vector carrying herpes simplex virus thymidine kinase gene under the control of human α-fetoprotein gene promoter. Cancer Res., 55: 3105-3109, 1995.


Fujita M, Murata K, Shiku H. Selective inhibition of human T-lymphotropic virus type I-transformed human T-cell growth by a tax-targeted conditionally cytotoxic recombinant retrovirus.  Blood, 84: 2591-2596, 1994.



英文著書

Fujita MDNA Replication Initiation.  In: Ganten D., Ruckpaul K, editors. Encyclopedic Reference of Genomics and Proteomics in Molecular Medicine. Springer, Berlin Heidelberg & New York: pp446-449, 2006.



英文総説

Sugimoto, N. and Fujita, M. Molecular mechanism for chromatin regulation during MCM loading in mammalian cells. DNA replication: From Old Principles to New Discovery. Adv. Exp. Med. Biol., (Springer, Masai, H. and Foiani, M Eds), 1042: 61-78, 2017.


Takafuji,T.  Kayama, K., Sugimoto, N., and Fujita, M. GRWD1, a new player among oncogenesis-related ribosonal/ nucleolar proteins. Cell Cycle 16: 1397-1403, 2017.


Higa, M., Fujita, M., Yoshida, K.  DNA Replication Origins and Fork Progression at Mammalian Telomeres. Genes,8(4): 2017.


Yoshida K, Lengronne A, Pasero P. The replication timing program in the hands of two HDACs. Microbial Cell,1: 273-275,2014.


Yoshida K, Poveda A, Pasero P.,Time to be versatile:regulation of the replication timing program in budding yeast. J Mol Biol., 425: 4696-4705, 2013.


Mechali M.,Yoshida K.,Coulombe P.,Pasero P.:Genetic and epigenetic determinants of DNA replication origins, position and activation. Curr Opin Genet Dev. 23:124-31,2013.


Fujita M.  Cdt1 revisited: complex and tight regulation during the cell cycle and consequences of deregulation in mammalian cells.  Cell Div., 1: 22, 2006.


Tsurumi T, Fujita M, Kudoh, A.  Latent and lytic Epstein-Barr virus replication strategies. Rev. Med. Virol., 15: 3-15, 2005.


Tsurumi T, Fujita M.  Molecular basis for Epstein-Barr virus DNA replication. Curr. Top. Virol., 2: 85-100, 2002.


Fujita M.  Cell cycle regulation of DNA replication initiation proteins in mammalian cells. Front Biosci., 4: 816-823, 1999.



和文総説

吉田和真. 出芽酵母においてヒストン脱アセチル化酵素はリボソームDNA領域におけるクロマチンの制御を介して複製のタイミングを制御している. 「ライフサイエンス 新着論文レビュー」, 2014. http://first.lifesciencedb.jp/archives/8926(別ウィンドウが開きます)


藤田雅俊. 染色体動態制御における複製開始複合体因子の新たな機能. 蛋白質核酸酵素増刊「染色体サイクル」 54:531-536, 2009.


藤田雅俊. 概論:染色体動態制御と疾患の研究における新展開. 蛋白質核酸酵素増刊「染色体サイクル」 54:552-555, 2009.


藤田雅俊. DNA複製因子とがん. 実験医学増刊「染色体サイクル」:191-196, 2007.


藤田雅俊. サイクリン/CDKから見た複製開始タンパク質の細胞周期調節. 実験医学 20: 540-545, 2002.


藤田雅俊. 哺乳動物細胞における複製開始タンパク質の機能と細胞周期調節. 実験医学増刊「細胞周期研究のフロンティア」:103-109, 2000.


藤田雅俊. 培養細胞における細胞周期の同調法. 実験医学 18: 74-76, 2000.


藤田雅俊、珠玖 洋. アンチセンスDNAによるウイルスの制御. 炎症と免疫 4: 164-169, 1996.


藤田雅俊、村田 健、珠玖 洋. 癌抑制遺伝子を用いた遺伝子治療の可能性. Biomedical Perspectives 2: 195-202, 1993.